The disease was named for the characteristic striping and discoloration in potato chips produced from infected tubers, but it affects all market classes of potato, by reducing both yield. Cts 0394917 department of agriculture and fisheries biosecurity act 2014 area freedom certificate i certify that to the best of my knowledge the area described below of the state of queensland is free of the bacterium candidatus liberibacter solanacearum syn. Candidatus liberibacter solanacearum associated with bactericera trigonicaaffected carrots in. Draft pra report for candidatus liberibacter psyllaurous summary 7 summary biosecurity australia is undertaking a pest risk analysis to assess the quarantine risks posed by candidatus liberibacter psyllaurous, which infects solanaceous crops, including tomato, capsicum, eggplant, potato and tamarillo. Rapid pest risk analysis for candidatus liberibacter. The asian hlb strain, candidatus liberibacter asiaticus is more heat tolerant, while the african strain, candidatus liberibacter africanus is asymptomatic at temperatures above 30c.
The closelyrelated bacteria candidatus liberibacter solanacearum clso, associated with vegetative disorders in carrots, is transmitted by the carrot psyllid bactericera trigonica. Piersonmethods for rapid and effective pcrbased detection of candidatus liberibacter solanacearum from the insect vector bactericera cockerelli. It describes how the plant health service for england will respond if the solanaceous haplotypes of candidatus liberibacter solanacearum lso andor their psyllid vector bactericera cockerelli, which together constitute the zebra chip complex, are discovered on. Maestro lot 302012 after 90 days reaching 12% after 150 days cultivation. Liefting lw, veerakone s, ward li, clover grg 2009 first report of candidatus phytoplasma australiense in potato. Genomes of candidatus liberibacter solanacearum haplotype a. The disease was first reported in the united states in 2004. This document is pp320, one of a series of the plant pathology department, ufifas extension. Like other members of liberibacter, lsozc1 encodes a flagellin domaincontaining protein fla lso with a conserved 22 aminoacid peptide flg22 lso. Candidatus liberibacter solanacearum edis university of florida. The bacterium is a carrot seed borne pathogen that can also be transmitted from potato mother tubers and by psyllid vectors. Sep, 2017 the occurrence of candidatus liberibacter solanacearum lso was determined in commercial and wild species of the family apiaceae, obtained from three seed collections held in the united kingdom.
Candidatus liberibacter solanacearum revision history. Draft pra report for candidatus liberibacter psyllaurous summary 7 summary biosecurity australia is undertaking a pest risk analysis to assess the quarantine risks posed by candidatus liberibacter psyllaurous, which infects solanaceous crops, including tomato, capsicum, eggplant, potato and. Liberibacter solanacearum in spain transmission trials. First report of candidatus liberibacter asiaticus in diaphorina communis 3 martinez al, wallace jm 1967 citrus leafmottleyellows disease in the philippines and transmission of the. Organization of the candidatus liberibacter solanacearum genome and identification of prophagelike regions while ca. Candidatus liberibacter solanacearum is a phloemlimited, gramnegative. This pest data sheet provides basic scientific information about candidatus liberibacter solanacearum and the damage it can cause. This bacterium is closely related to other pathogenic candidatus liberibacter spp. Candidatus liberibacter solanacearum, associated with. Pdf first report of candidatus liberibacter solanacearum. Three batches of carrot seeds harvested in three infected carrot fields in france and one batch of. Rapid pest risk analysis for candidatus liberibacter solanacearum this document provides a rapid assessment of the risks posed by the pest to the uk in order to assist risk managers decide on a response to a new or revised pest threat. Pitman ar, drayton gm, kraberger sj, genet ra, scott ia 2011 tuber transmission of candidatus liberibacter solanacearum and its association with zebra chip on potato in new zealand. Pest survey card on candidatus liberibacter solanacearum.
Detection of candidatus liberibacter solanacearum in. Candidatus liberibacter is transmitted by two insects from psyllidae family diaphorina citri in asia, brazil and florida, and trioza erytreae in africa. Multiplex realtime pcr for detection, identification and. Pcrbased diagnostic methods for candidatus liberibacter. This documentpublication is also available on our website at. Documents about candidatus liberibacter solanacearum libeps. Pest information candidatus liberibacter solanacearum is a phloemlimited, gramnegative, unculturable bacterium that is associated with several emerging diseases. Draft annex to ispm 27 candidatus liberibacter solanacearum page 2 of 42 international plant protection convention for the identification of candidatus liberibacter solanacearum diagnostic protocol as there is only one method and there are no decision points. Candidatus liberibacter solanacearum lso is associated with disease in apiaceous and solanaceous hosts. The bacterium is a carrot seed borne pathogen that can also be transmitted. A rapid field detection system for citrus huanglongbing. Rapid pest risk analysis for candidatus liberibacter solanacearum. Candidatus liberibacter solanacearum is also associated with other significant tomato, capsicum and tamarillo diseases liefting et al.
Introduction the symptoms on peppers include stunted growth, and. Introduction the symptoms on potatoes can go unnoticed until the potato tubers are. First report of candidatus liberibacter solanacearum in carrot in france. This document is pp320, one of a series of the plant pathology department, ufifas extensi. Mar 14, 2020 candidatus liberibacter solanacearum is a gram. Candidatus liberibacter solanacearum was detected in 5% of seedlings cv. Pdf three haplotypes of the recently discovered bacterium species candidatus liberibacter solanacearum are described and related to geographic. Candidatus liberibacter asiaticus accumulates inside.
First report of candidatus liberibacter solanacearum in carrot in mainland spain. Risk assessment of candidatus liberibacter solanacearum. Pdf first report of candidatus liberibacter asiaticus in. Liberibacter solanacearum by bactericera trigonica g. The national regulatory control system pm 925 1 bactericera cockerelli and candidatus liberibacter solanacearum provides guidance on excluding b. Candidatus liberibacter solanacearum libepsoverview. Marianne loiseau 1, soraya garnier2, violaine boirin3, maryse merieau4, anne leguay1, isabelle renaudin 1, jeanphilippe renvoise 2, pascal gentit 1, francoise. The initial round of sequencing was done using the flx standard pyrosequencing method. Nz082226 represents a novel candidate species of candidatus liberibacter, for which the name candidatus liberibacter solanacearum is proposed. Pm100228 eppo a1 and a2 lists of pests recommended for regulation as quarantine pests 2019.
Zebra chip disease is found in all states west of the rocky mountains, kansas and in texas. Pm 7143 1 candidatus liberibacter solanacearum 2020. Alfarofernandez a, siverio f, cebrian mc, villaescusa fj, font mi, 2012a. Candidatus liberibacter solanacearum sequence generation and assembly.
Candidatus liberibacter solanacearum lso status full name candidatus liberibacter solanacearum lso is an emerging threat to a range of crops, most notably potato, tomato, carrot and celery currently there are 7 described lso haplotypes genetic variants. Candidatus liberibacter solanacearum lso is a phloemrestricted plant pathogen that is vectored between host plants by the potato psyllid, bactericera cockerelli. Candidatus liberibacter psyllaurous draft pra 20090506. Candidatus liberibacter asiaticus and candidatus liberibacter americanus are transmitted by the adults of the citrus psyllid diaphorina citri kuwayana. Candidatus liberibacter asiaticus calas, a noncultured member of the. Recently, lso has been recorded in the uk in psyllid species not previously known to harbour the bacterium. Pdf first report of candidatus liberibacter asiaticus. Candidatus liberibacter solanacearum, associated with plants. Liberibacter asiaticus and liberibacter americanus are transmitted by the adults of the citrus psyllid d. Although only small quantities of each seed accession was tested, a high proportion of commercial carrot seed lots.
The specificity and sensitivity of polymerase chain reaction pcr primers developed for candidatus liberibacter solanacearum and candidatus liberibacter psyllaurous were evaluated in. May 21, 2014 candidatus liberibacter solanacearum was detected in 5% of seedlings cv. This document is part of a toolkit that consists of pest specific documents, such. Candidatus liberibacter solanacearum agriculture and food. Supplementary file s2 and five nucleotide differences in the 16s23s intergenic spacer region. Zebra chip disease of potato is caused by the bacterium candidatus liberibacter solanacearum clso and is a serious pest of potato in the americas and new zealand. First report of candidatus liberibacter solanacearum. Pdf transmission of candidatus liberibacter solanacearum. Candidatus liberibacter solanacearum causes zebra chip disease of potato. Induction of innate immune responses by flagellin from the. Although only small quantities of each seed accession was tested, a high proportion of commercial. In this version an internal amplification control is added to the seqpcr background in ishivegs view there is a lack of evidence for seed transmission of candidatus liberibacter solanacearum ca. In the open field plot the same plants of the same cultivar were 2% infected after 90 days and 96% infected after 180 days. Candidatus liberibacter solanacearum is an exotic pest to australia.
The bacterium candidatus liberibacter solanacearum is a recent plant pathogen of several crops in solanaceae and apiaceae and is associated with economically important diseases. An emerging pathogen infecting potato and tomato1 binoy babu, mathews l. Pdf haplotypes of candidatus liberibacter solanacearum. Dp 21 diagnostic protocols for regulated pests dp 212 international plant protection convention 1. Candidatus liberibacter solanacearum lso is an emergent pathogen of carrots in europe and solanaceous plants in north and central america and new zealand. Economic justification for quarantine status the case. A rapid field detection system for citrus huanglongbing associated candidatus liberibacter asiaticus from. Candidatus liberibacter solanacearum zebra chip cabi. How vector sex influences lso transmission has not been yet elucidated. For instance, in texas, the annual potato yield loss caused by ca. Candidatus liberibacter solanacearum lso is a pathogen of solanaceae but also causes serious physiological disorders in carrots and celery apiaceae. It does not constitute a detailed pest risk analysis pra but includes.
First report of candidatus liberibacter solanacearum associated with the psyllid bactericera trigonica hodkinson on carrots in northern africa. Candidatus liberibacter solanacearum is a bacterial plant pathogen that is exotic to australia. Localization and distribution of candidatus liberibacter. The candidate genus candidatus liberobacter was first proposed by jagoueix et al. Following the recent detections of candidatus liberibacter solanacearum on carrots in europe and particularly in fields used for seed production in france in 2012, experiments to study the transmission of this pathogen by seeds were conducted. Candidatus liberibacter solanacearum is found in all states west of the rocky mountains, kansas and in texas. Candidatus liberibacter solanacearum lso, which is vectored by the potato psyllid bactericera cockerelli. Binoy babu, 09 2014 detection and identification of. Candidatus liberibacter solanacearum jennifer sjolund, wendy monger, mairi carnegie, alex greenslade, colin jeffries, david kenyon, james bell, fiona highet candidatus liberibacter solanacearum lso is an insectvectored bacterium that colonises plant phloem and causes the devastating zebra chip disease in potato. The complete genome sequence of candidatus liberibacter. The eppo diagnostic protocol differs in terms of format but it is consistent with the content of the ippc standard. This pest specific response plan has been prepared by the defra risk and policy team.
Department of agriculture and fisheries biosecurity act. The occurrence of candidatus liberibacter solanacearum lso was determined in commercial and wild species of the family apiaceae, obtained from three seed collections held in the united kingdom. Detection of candidatus liberibacter solanacearum in carrot seed. The accessions dated from the 1970s and the seed had been sourced worldwide. Detection, distribution, and genetic variability of.
Candidatus liberibacter solanacearum clso is an exotic pest to australia. Transmission of candidatus liberibacter solanacearum by. To understand the innate immune responses triggered by this. A novel haplotype of candidatus liberibacter solanacearum found. Candidatus liberibacter solanacearum lso is a phloemlimited, gramnegative, unculturable bacterium that is primarily spread by psyllid insect. Economic justification for quarantine status the case study. General information about candidatus liberibacter solanacearum libeps eppo global database. Two rounds of 454 pyrosequencing were carried out to obtain the complete ca.
Liberibacter solanacearum by bactericera trigonica field surveys. Binoy babu, 09 2014 1 detection and identification of candidatus liberibacter solanacearum in potato and tomato a new bacterial species candidatus liberibacter solanacearum has been found in association with serious diseases of tomatoes, potatoes and other solanaceous crops observed. It is now apparent that candidatus liberibacter solanacearum and candidatus liberibacter psyllaurous do not represent different species, since their 16s rrna gene sequence shows a 99. A survey of candidatus liberibacter solanacearum in. Candidatus liberibacter solanacearum infects pepper plants. Liberibacter solanacearum lso andor their psyllid vector bactericera. Candidatus liberibacter solanacearum lso is bacterium transmitted by psyllids to solanaceae and apiaceae plants. Candidatus liberibacters are gramnegative bacteria with a doublemembrane cell envelope found in the sieve tube elements of phloem. Candidatus liberibacter solanacearum libepsdocuments. Documents about candidatus liberibacter solanacearum libeps number title download. It can affect both solanaceous and apiaceous crops. In order to understand the molecular interaction of this pathogen and its psyllid vector, bactericera cockerelli, illumina. Candidatus liberibacter solanacearum associated with bactericera trigonicaaffected carrots in the canary islands. Due to the difficulties of in vitro culture, antibodies against calas have not been widely used in studies of this pathogen.
In carrots, this pathogen is transmitted by the psyllids bactericera trigonica and trioza apicalis. Candidatus liberibacter solanacearum charakteristika a biologia vektor bactericera cockerelli je znamy prenosom cls na rastlinach zemiaka a rajciaka v usa, mexiku, strednej amerike guatemala, honduras, nikaragua a na novom zelande povodne. Multiplex realtime pcr for detection, identification and quantification of candidatus liberibacter solanacearum in potato plants with zebra chip. The bacteria are transmitted by psyllids as they feed. It is restricted to the host plants phloem and psyllid vectors hemolymph, alternating its life cycle between host plants and insect vectors. Transmission of candidatus liberibacter solanacearum in. Genomes of candidatus liberibacter solanacearum haplotype a from new zealand and the united states suggest significant genome plasticity in the species sarah m. Pest information candidatus liberibacter solanacearum is a phloemlimited, gramnegative, unculturable bacterium.
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